更新時間：2022-08-25
一氧化氮廣泛分布于生物體內(nèi)各組織中，特別是神經(jīng)組織中。它是一種新型生物信使分子。NO是一種極不穩(wěn)定的生物自由基，分子小，結(jié)構(gòu)簡單，常溫下為氣體，微溶于水，具有脂溶性，可快速透過生物膜擴散，生物半衰期只有3-5秒，其依賴于一氧化化氮合成酶(nitric oxide synthase , NOS)并在心、腦血管調(diào)節(jié)、神經(jīng)、免疫調(diào)節(jié)等方面有著十分重要的生物學(xué)作用。
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一氧化氮比色分析試劑盒提供了一個只需要兩部，便可準(zhǔn)確便捷的檢測檢測亞硝酸鹽離子和硝酸鹽離子濃度的方案。*步，硝酸鹽離子被硝酸鹽還原酶轉(zhuǎn)化成亞硝酸鹽離子，然后，使用Griess試劑將亞硝酸鹽轉(zhuǎn)化成深紫色的含氮化合物。該含氮化合物內(nèi)發(fā)色團(tuán)的量反應(yīng)了檢測樣本內(nèi)NO的總量。該試劑盒檢測靈敏度為0·1nmole 亞硝酸鹽/孔或1μM亞硝酸鹽。該一氧化氮熒光分析試劑盒可用于哺乳動物細(xì)胞組織裂解液、細(xì)胞培養(yǎng)上清、血漿、血清、尿液以及組織勻漿內(nèi)一氧化氮含量的檢測。
如需了解人ELISA試劑盒價格是否有折扣、*或是否有調(diào)整,請咨詢我司業(yè)務(wù)人員:
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Kit Summary:
• Detection method- Absorbance （540 nm）
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.
Features & Benefits:
• Simple two-step procedure; takes only ~1-1.5 hours
• Fast and convenient
• BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite.
Kit components:
• Assay Buffer
• Enzyme cofactor
• Enhancer
• Nitrate Reductase
• Nitrate Standard
• Nitrite Standard
• Griess Reagent R1
• Griess Reagent R2
• Microtiter Plate
• Plate Cover
Description:
Nitric oxide （NO） plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production. BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accuray reflects nitric oxide amount in samples. The detection limit of the assay is approximay 0.1 nmole nitrite/well, or 1 µM.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
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